Determination of Mycotoxin Metabolites

DON was obtained from Romer Labs (Tulln, Austria) and CER was purified as previously described [28 (link)]. Cell culture media and supplements were obtained from Gibco® Life Technologies (Karlsruhe, Germany). KH₂PO₄, KCl, NaCl, Na₂HPO₄, D-glucose, HEPES, CaCl₂, MgCl₂, glycin, formaldehyde, sodium dodecyl sulfate (SDS), NaOH and Triton X-100 were purchased from Carl Roth (Karlsruhe, Germany) and Lucifer Yellow CH di-lithium salt from Santa Cruz Technologies (Dallas, TX, USA). For LC-MS/MS measurements, MS grade water was purchased from VWR (Fontenay-sous-Bois, France), acetonitrile (ACN) and methanol (MeOH) from Honeywell (Seelze, Germany). Acetic acid was obtained from Merck (Darmstadt, Germany) and ammonium acetate (both LC-MS grade) from Sigma Aldrich (Vienna, Austria). DON-3-sulfate, DON-15-sulfate and DON-3-glucuronide were kindly provided by Dr. Philipp Fruhmann (Technical University of Vienna) and synthesized as previously described [44 (link)].

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Beisl J., Varga E., Braun D., Warth B., Ehling-Schulz M., Del Favero G, & Marko D. (2021). Assessing Mixture Effects of Cereulide and Deoxynivalenol on Intestinal Barrier Integrity and Uptake in Differentiated Human Caco-2 Cells. Toxins, 13(3), 189.

Publication 2021

Cell culture media and supplements KH2PO4 KCl NaCl Na2HPO4 D-glucose HEPES CaCl2 MgCl2 glycin formaldehyde sodium dodecyl sulfate NaOH Triton X-100 MS grade water acetonitrile (ACN) methanol (MeOH) Acetic acid ammonium acetate

Acetic acid Acetonitrile Ammonium acetate Cell Cell culture Culture media Formaldehyde Glucose Glucuronide Glycin Hepes Lithium Lucifer yellow ch Methanol Mgcl2 Ms ms Salt Sodium dodecyl sulfate Sulfate Supplements Triton x 100

Corresponding Organization : University of Vienna

Other organizations : University of Veterinary Medicine Vienna

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Variable analysis

independent variables

DON obtained from Romer Labs (Tulln, Austria)
CER purified as previously described [28 (link)]

dependent variables

Not explicitly mentioned

control variables

Cell culture media and supplements obtained from Gibco® Life Technologies (Karlsruhe, Germany)
KH2PO4, KCl, NaCl, Na2HPO4, D-glucose, HEPES, CaCl2, MgCl2, glycin, formaldehyde, sodium dodecyl sulfate (SDS), NaOH and Triton X-100 purchased from Carl Roth (Karlsruhe, Germany)
Lucifer Yellow CH di-lithium salt from Santa Cruz Technologies (Dallas, TX, USA)
For LC-MS/MS measurements, MS grade water from VWR (Fontenay-sous-Bois, France), acetonitrile (ACN) and methanol (MeOH) from Honeywell (Seelze, Germany), acetic acid from Merck (Darmstadt, Germany) and ammonium acetate (both LC-MS grade) from Sigma Aldrich (Vienna, Austria)

controls

DON-3-sulfate, DON-15-sulfate and DON-3-glucuronide kindly provided by Dr. Philipp Fruhmann (Technical University of Vienna) and synthesized as previously described [44 (link)]

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