The Balb/3T3 and NIH/3T3 fibroblast cells were cultured in Dulbecco’s modified
Eagle medium high-glucose (DMEM-HG; Gibco, Carlsbad, CA, USA) supplemented with
10% fetal bovine serum (FBS, Gibco) and 1% antimycotic solution (Gibco) in a 5%
CO2 humidified atmosphere at 37°C.40 (link),41 (link) Each cells were seeded at
a density of 3 × 103 cells/well on 96 well plates and incubated for
24 h. The HCD matrix containing different concentration ratios of the S-EGF and
S-bFGF were added in medium of each well and the cells were subsequently starved
in serum-free DMEM medium for 72 h. Then thyiazolyl blue tetrazolium bromide
(MTT, M2128-100MG; St. Louis, MO, USA Sigma-Aldrich) was added. After 1.5 h of
incubation, the absorbance was read at 450 nm.42 (link)
Eagle medium high-glucose (DMEM-HG; Gibco, Carlsbad, CA, USA) supplemented with
10% fetal bovine serum (FBS, Gibco) and 1% antimycotic solution (Gibco) in a 5%
CO2 humidified atmosphere at 37°C.40 (link),41 (link) Each cells were seeded at
a density of 3 × 103 cells/well on 96 well plates and incubated for
24 h. The HCD matrix containing different concentration ratios of the S-EGF and
S-bFGF were added in medium of each well and the cells were subsequently starved
in serum-free DMEM medium for 72 h. Then thyiazolyl blue tetrazolium bromide
(MTT, M2128-100MG; St. Louis, MO, USA Sigma-Aldrich) was added. After 1.5 h of
incubation, the absorbance was read at 450 nm.42 (link)
