Protocol detail

Cell Viability Assay with MTT

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Cells were plated onto 96-well plates at seeding densities of 6.5 × 10³ cells per well for PA-1, H1299 and SiHa cells and 7 × 10³ cells per well for D54MG cells. The cell viability after treatment with appropriate agents was measured using Thiazolyl Blue Tetrazolium Bromide (MTT, Sigma) as previously described [17 (link)]. Concentrations of DHA that produced 50% inhibition in cell survival (IC₅₀) following a 24 h exposure, were manually derived from dose–response curves generated by the Microsoft Excel 2010 edition.

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Jeong S., Jing K., Kim N., Shin S., Kim S., Song K.S., Heo J.Y., Park J.H., Seo K.S., Han J., Wu T., Kweon G.R., Park S.K., Park J.I, & Lim K. (2014). Docosahexaenoic acid-induced apoptosis is mediated by activation of mitogen-activated protein kinases in human cancer cells. BMC Cancer, 14, 481.

Publication 2014

Thiazolyl Blue Tetrazolium Bromide (MTT,

After treatment Cell survival Cells Inhibition Thiazolyl blue tetrazolium bromide

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Variable analysis

independent variables

Concentrations of DHA

dependent variables

Cell viability
IC50 (Concentration of DHA that produced 50% inhibition in cell survival)

control variables

Seeding densities of cells (6.5 × 10^3 cells per well for PA-1, H1299 and SiHa cells, and 7 × 10^3 cells per well for D54MG cells)
Treatment duration (24 h)

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