Western Blot Analysis of Protein Expression

Western blotting was performed essentially as previously described (17 (link)). The expression of CD137 (1:500, rat, Abcam, Cambridge, UK), ASK1 (1:2,000, rabbit, Abcam), phospho-ASK1 [1:500, rabbit, Cell Signaling Technology (CST), Danvers, MA, USA], p38 (1:1,000, rabbit, CST), phospho-p38 (1:1,000, rabbit, CST), JNK (1:1,000, rabbit, CST), phospho-JNK (1:1,000, rabbit, CST), IκBα (1:1,000, rabbit, CST), phospho-IκBα (1:1,000, rabbit, CST), MMP9 (1:1,000, rabbit, Abcam), MMP12 (1:2,000, rabbit, Abcam), and β-actin (1:1,000, rabbit, CST) in MH-S cells or lung tissues was measured by western blot. Protein expression was normalized to β-actin.

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Lu Y., Li C., Du S., Chen X., Zeng X., Liu F., Chen Y, & Chen J. (2018). 4-1BB Signaling Promotes Alveolar Macrophages-Mediated Pro-Fibrotic Responses and Crystalline Silica-Induced Pulmonary Fibrosis in Mice. Frontiers in Immunology, 9, 1848.

Publication 2018

phospho-ASK1 phospho-p38 phospho-JNK phospho-IκBα MMP9 MMP12 β-actin

Actin Ask1 Cd137 Cells Lung Mmp9 Phospho Protein Rabbit Tissues Western blot

Corresponding Organization :

Other organizations : China Medical University

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Variable analysis

independent variables

Western blotting protocol

dependent variables

Expression of CD137
Expression of ASK1
Expression of phospho-ASK1
Expression of p38
Expression of phospho-p38
Expression of JNK
Expression of phospho-JNK
Expression of IκBα
Expression of phospho-IκBα
Expression of MMP9
Expression of MMP12

control variables

β-actin expression

Annotations

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