Quantification of Endothelial Progenitor Cells

The number of EPCs was tested by fluorescence-activated cell sorting (BD LSR II with FACS Diva Software, BD Biosciences, USA). Two hundred microliters of whole blood was transferred to polystyrene tubes for testing with the following antibodies added to the samples: APC-conjugated anti-human CD45, FITC-conjugated anti-human CD34 (BD Biosciences, USA), and PE-conjugated anti-human KDR/VEGF-2 (BD Biosciences, USA). The experimental protocol can be found elsewhere (13 (link)).

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Zhen X., Zheng Y., Hong X., Chen Y., Gu P., Tang J., Cheng H., Yuan T.F, & Lu X. (2016). Physiological Ischemic Training Promotes Brain Collateral Formation and Improves Functions in Patients with Acute Cerebral Infarction. Frontiers in Neurology, 7, 235.

Publication 2016

BD LSR II FACS Diva Software APC-conjugated anti-human CD45 FITC-conjugated anti-human CD34

Antibodies Blood Epcs Fitc Human Polystyrene Vegf

Corresponding Organization : Nanjing Normal University

Other organizations : China Rehabilitation Research Center, Capital Medical University, Hong Kong Polytechnic University, Sichuan University, West China Hospital of Sichuan University

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Variable analysis

independent variables

The number of EPCs (Endothelial Progenitor Cells)

dependent variables

EPCs measured by fluorescence-activated cell sorting (BD LSR II with FACS Diva Software, BD Biosciences, USA)

control variables

200 microliters of whole blood transferred to polystyrene tubes
APC-conjugated anti-human CD45
FITC-conjugated anti-human CD34 (BD Biosciences, USA)
PE-conjugated anti-human KDR/VEGF-2 (BD Biosciences, USA)

controls

The experimental protocol can be found elsewhere (13 (link))

Annotations

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