Mosquito Malaria Infection Analysis

A. gambiae s.s. mosquitoes (Yaoundé strain) were reared and maintained as described previously [17 (link)]. Three to four day old female mosquitoes were cold-anaesthetized and inoculated intrathoracically with 69 nl of a 50, 100 or 200 μg/ml solution of sHz or with the same volume of endotoxin-free PBS, using a Nanoject micro-injector (Drummond Scientific). Mosquitoes were left to rest for 24 h. Female CD1 mice were intraperitoneally inoculated with 10⁷Plasmodium berghei (ANKA) GFPcon 259 cl2 parasitized red blood cells/ml and mosquitoes were fed as previously optimized in our Lab [17 (link)]. Four independent biological replicates were performed for each experiment. Between 8 and 10 days post-infection, mosquito midguts were collected to determine infection rate (prevalence) and intensity.