Arsenic species, including trivalent and pentavalent forms of 2 and 1 , were analyzed by high-pressure liquid chromatography (HPLC) (Series 2000; PerkinElmer, Waltham, MA, USA) coupled to inductively coupled plasma mass spectrometry (ICP-MS) (ELAN DRC-e; PerkinElmer), as described previously,3 ,5 (link) with minor modifications. Briefly, arsenic species in samples were separated by HPLC on a BioBasic 18 LC column (250 mm × 4.6 mm, 5 μm, 300 Å) (Thermo Fisher Scientific) using a mobile phase consisting of 3 mM malonic acid and 5% MeOH (v/v) (pH 5.6 adjusted with tetrabutylammonium hydroxide) with a flow rate of 1 mL min−1 at 25 °C. Arsenic was monitored by ICP-MS. Arsenic species were determined from the HPLC retention time of known standards (Figures 1 and 2 ).
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Arsenic Speciation Analysis by HPLC-ICP-MS
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Arsenic
High pressure liquid chromatography
Malonic acid
Mass spectrometry
Plasma
Retention
Tetrabutylammonium hydroxide
Corresponding Organization : Florida International University
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Variable analysis
independent variables
- Arsenic species, including trivalent and pentavalent forms
- Arsenic species concentrations
- Mobile phase composition (3 mM malonic acid and 5% MeOH (v/v), pH 5.6 adjusted with tetrabutylammonium hydroxide)
- HPLC flow rate (1 mL min^-1)
- HPLC column (BioBasic 18 LC column, 250 mm × 4.6 mm, 5 μm, 300 Å)
- HPLC temperature (25 °C)
- Known standards for arsenic species identification (Figures 1 and 2)
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