Protocol detail

Luciferase Assay for Hedgehog Signaling

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Myers B.R., Sever N., Chong Y.C., Kim J., Belani J.D., Rychnovsky S., Bazan J.F, & Beachy P.A. (2013). Hedgehog Pathway Modulation by Multiple Lipid Binding Sites on the Smoothened Effector of Signal Response. Developmental cell, 26(4), 346-357.

Publication 2013

Agonists Alkaline phosphatase Allele Antagonists Assay Baculovirus Cdna Cells Conditioned medium Dish Hek293 cells Light Lipofectamine 2000 Lovastatin Luciferase Nih3t3 Plasmid Promega Renilla Sapphire Serum Sf9 cells Sodium butyrate Sterols Sv40 Transfection

Corresponding Organization :

Other organizations : Stanford University, Howard Hughes Medical Institute, University of California, Irvine, University of Minnesota

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