Comprehensive miRNA and mRNA Expression Analysis

Comprehensive microRNA expression analysis was performed using the 3D-Gene human microRNA oligo chip (Toray Industries, Inc., Japan), which was designed to detect 2,565 microRNAs registered in miRBase release 21 (http://www.mirbase.org/). Similarly, comprehensive mRNA expression analysis was performed using the 3D-Gene human oligo chip 25K manual (v. 2.01). The microarray data signals were treated similarly as previously described [3 (link)]. To normalize signals across the different microarrays, quantile normalization was applied. Numeric analyses were performed using R v. 3.1.2 (R foundation for Statistical Computing, http://www.R-project.org). All microarray data from this study are in agreement with the Minimum Information about a Microarray Experiment standard and are publicly available through the Gene Expression Omnibus database (http://www.ncbi.nlm.nih.gov/projects/geo/) under accession number GSE114318.