NADPH Oxidase Activity Assay

The enhanced lucigenin-derived chemiluminescence method was adopted to measure the nicotinamide adenine dinucleotide phosphate (NADPH) oxidase activity and ROS level in aortic tissues or A7r5 cells. Both dark-adapted 100 μM NADPH and 5 μM lucigenin were used to trigger the photon emission and the background chemiluminescence was detected by a luminometer (Turner, CA, USA). The data were presented as the mean of light unit (MLU)/min/mg protein.

Free full text: Click here

Wang H.Y., Wang F.Z., Chang R., Wang Q., Liu S.Y., Cheng Z.X., Gao Q., Zhou H, & Zhou Y.B. (2023). Adrenomedullin Improves Hypertension and Vascular Remodeling partly through the Receptor-Mediated AMPK Pathway in Rats with Obesity-Related Hypertension. International Journal of Molecular Sciences, 24(4), 3943.

Publication 2023

Aortic Cells Chemiluminescence Light protein Lucigenin Nicotinamide adenine dinucleotide phosphate Nicotinamide adenine dinucleotide phosphate oxidase Tissues Trigger

Corresponding Organization : Nanjing Medical University

Top 5 similar protocols

Variable analysis

independent variables

NADPH concentration (100 μM)
Lucigenin concentration (5 μM)

dependent variables

NADPH oxidase activity
ROS level

control variables

Aortic tissues or A7r5 cells

controls

Dark-adapted conditions

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!