Serum Copper and Zinc Quantification

A validated and clinically available laboratory developed test using Inductively Coupled Plasma Mass Spectrometry (ICP-MS) was used to measure copper and zinc in bulk serum. A PerkinElmer Elan or PerkinElmer NexION 350D ICP-MS spectrometer (Waltham MA) was used for all analyses. This assay used six calibration standards to establish an analytical measurement range of 0–5,000 ng/ml for both copper and zinc, respectively. Utak Trace Elements Serum Toxicology controls (Valencia, CA) were analyzed with each calibration and sample run to monitor the accuracy of the method. This assay was developed, and the performance characteristics were established in a manner to meet Clinical Laboratory Improvement Amendments (CLIA) requirements. A detailed description of the ICP-MS method used for the quantification of total copper and zinc in the serum of these samples has been described in a previous publication by our group (Mehta et al., 2021 (link)).

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Miller K., Day P.L., Behl S., Stromback L., Delgado A., Jannetto P.J., Wieser M.E., Mehta S.Q, & Pandey M.K. (2023). Isotopic composition of serum zinc and copper in healthy children and children with autism spectrum disorder in North America. Frontiers in Molecular Neuroscience, 16, 1133218.

Publication 2023

NexION 350D ICP-MS spectrometer

Assay Bulk Clinical laboratory Copper Mass spectrometry Plasma Serum Trace elements Zinc

Corresponding Organization : Mayo Clinic in Arizona

Other organizations : Mayo Clinic in Florida, Mayo Clinic, WinnMed

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Copper concentration in bulk serum
Zinc concentration in bulk serum

control variables

Inductively Coupled Plasma Mass Spectrometry (ICP-MS) spectrometer used for all analyses
Analytical measurement range of 0–5,000 ng/ml for both copper and zinc
Utak Trace Elements Serum Toxicology controls analyzed with each calibration and sample run to monitor the accuracy of the method

controls

Positive control: Utak Trace Elements Serum Toxicology controls
Negative control: Not specified

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