HepG2 cells (Cell Bank of Chinese Academy of Sciences) were suspended in DMEM (BI, Israel) containing 10% fetal bovine serum (FBS; Gibco) and incubated at 37°C with 5% CO2. To evaluate the effect of luteolin on HepG2 cells, 5, 10, 20, 40, 80, 160, and 320 μmol/L luteolin (Sigma) was used. To determine the effect of ESR1 inhibition on HepG2 cells, 1, 20, 50, 100, 300, 500, and 900 nmol/L fulvestrant (Selleck China) was used. To determine the effect of Akt and MAPK-JNK signaling on ESR1 expression, Akt agonist SC79 (10 μmol/L, Selleck China), and MAPK-JNK agonist anisomycin (4 μmol/L, Selleck China) were used. DMSO served as a control.
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Luteolin and Estrogen Receptor Inhibition in HepG2 Cells
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Other organizations : Hainan Medical University
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Variable analysis
independent variables
- Luteolin concentration (5, 10, 20, 40, 80, 160, and 320 μmol/L)
- Fulvestrant concentration (1, 20, 50, 100, 300, 500, and 900 nmol/L)
- Akt agonist SC79 (10 μmol/L)
- MAPK-JNK agonist anisomycin (4 μmol/L)
- Effect of luteolin on HepG2 cells
- Effect of ESR1 inhibition on HepG2 cells
- Effect of Akt and MAPK-JNK signaling on ESR1 expression
- HepG2 cells (Cell Bank of Chinese Academy of Sciences)
- DMEM (BI, Israel) containing 10% fetal bovine serum (FBS; Gibco)
- Incubation at 37°C with 5% CO2
- DMSO as control
- DMSO as a control
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