Ten pairs of LUAD and adjacent normal tissues with complete clinicopathological information were collected from Huai’an First People's Hospital. The experiment was approved by the Medical Ethics Committee of Huai’an First People's Hospital, and each participant signed a written informed consent. The operation procedures of immunohistochemistry were reported in previous study.[10 (link)] All sections were incubated overnight at 4°C with anti-UBE2C (abcam, 1:300 dilution). Each immunostained section was independently read by 2 pathologists who did not know the clinicopathological data. The criteria for judging the results of immunohistochemistry has been described in previous study.[10 (link)] Wilcoxon signed-rank test was used to analyze the expression of UBE2C in LUAD and its adjacent tissues.
Protocol detail
Immunohistochemical analysis of UBE2C in LUAD
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Other organizations : Nanjing Medical University, Creative Commons, Huaian First People’s Hospital
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Variable analysis
independent variables
- Tissue type (LUAD vs. adjacent normal tissues)
- Expression of UBE2C protein
- Immunohistochemistry procedures (previously reported in the referenced study)
- Criteria for judging immunohistochemistry results (previously reported in the referenced study)
- Pathologists who read the immunostained sections (did not know the clinicopathological data)
- Not explicitly mentioned
- Not explicitly mentioned
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