Western Blot Analysis of p53, p21

Western blotting was performed using standard techniques previously described (27 (link)) and primary antibodies to anti-p53 (Santa Cruz Biotechnology, sc-126), anti-p21 (Calbiochem, OP64) and beta-actin (sc81178, Santa Cruz Biotechnology) were used.

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Neskey D.M., Osman A.A., Ow T.J., Katsonis P., McDonald T., Hicks S.C., Hsu T.K., Pickering C.R., Ward A., Patel A., Yordy J.S., Skinner H.D., Giri U., Sano D., Story M.D., Beadle B.M., El-Naggar A.K., Kies M.S., William W.N., Caulin C., Frederick M., Kimmel M., Myers J.N, & Lichtarge O. (2015). Evolutionary Action score of TP53 (EAp53) identifies high risk mutations associated with decreased survival and increased distant metastases in head and neck cancer. Cancer research, 75(7), 1527-1536.

Publication 2015

sc81178

Anti antibodies Beta actin

Corresponding Organization : The University of Texas MD Anderson Cancer Center

Other organizations : MUSC Hollings Cancer Center, Medical University of South Carolina, Albert Einstein College of Medicine, Yeshiva University, Baylor College of Medicine, Rice University, Dana-Farber Cancer Institute, The University of Texas Southwestern Medical Center, Tama University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Protein expression of p53, p21 and beta-actin

control variables

Not explicitly mentioned

controls

Positive control: None mentioned
Negative control: None mentioned

Annotations

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