Establishment and Culture of CM Cell Lines

The CM cell lines CRMM1, CRMM2, and CM2005.1 were originally established from recurrence patients [34 (link),35 (link)] and provided by the Liverpool Ocular Oncology Research Group. The authentication of the cell lines was performed using genotyping (Cellosaurus STR similarity search tool [36 (link)]) and negatively tested for mycoplasma infection using real time PCR [37 ]. Cells were cultured in complete medium containing F-12K medium with 10% FCS at 37 °C in a fully humidified atmosphere with 5% (v/v) CO₂. Spheroid generation was performed as described in previous publications [28 (link),38 (link)]. Briefly, spheroids were generated by seeding 5 × 10³ cells in round bottom 96-well ultralow attachment plates (Corning, Corning, NY, USA) containing 200 µL of complete medium.