A fluorescently labeled deoxyglucose analog, 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino]- 2-deoxy-glucose (2-NBDG) (Invitrogen #N13195, Waltham, MA, USA), was utilized as a probe for the detection of glucose uptake in cultured cells, as previously described [28 (link)]. Briefly, after 48 h transfection, cells were incubated with the 2-NBDG reagent (1 h). Cells were then trypsinized and analyzed by flow cytometry (BD FACS Aria III flow cytometer, San Jose, CA, USA) with excitation at 465 nm and emission at 540 nm.
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