C. adamantium O. Berg leaves and roots were collected following the identification of the plant and authorization of the SISBIO (Sistema de Autorização e Informação em Biodiversidade, permit number 54470).
Leaves and roots of C. adamantium O. Berg were collected in Dourados, in the state of Mato Grosso do Sul, Brazil (coordinates: 22° 02′ 47.9 S′ and 055° 08′ 14.3′ W). The samples were cleaned, dried in a convection oven at 45°C, and ground using a Croton knife mill. An exsiccated sample was deposited in the Herbarium of the Federal University of Grande Dourados, Mato Grosso do Sul, Brazil, with registration number 4108.
Extracts were prepared from the powdered material using an accelerated solvent extractor (ASE®-150, Dionex), as described by Espindola et al. (2016 (link)). The samples were placed in a cell of 100 mL and extracted with distilled water at temperature of 125°C through two 5 min static cycles, with an 80% flush volume and a 60 s purge. The extracts were lyophilized to obtain the dry extract. Thus, the aqueous extracts of Campomanesia leaves (AECL) and roots (AECR) were obtained with a yield of 13 and 6%, respectively.
Leaves and roots of C. adamantium O. Berg were collected in Dourados, in the state of Mato Grosso do Sul, Brazil (coordinates: 22° 02′ 47.9 S′ and 055° 08′ 14.3′ W). The samples were cleaned, dried in a convection oven at 45°C, and ground using a Croton knife mill. An exsiccated sample was deposited in the Herbarium of the Federal University of Grande Dourados, Mato Grosso do Sul, Brazil, with registration number 4108.
Extracts were prepared from the powdered material using an accelerated solvent extractor (ASE®-150, Dionex), as described by Espindola et al. (2016 (link)). The samples were placed in a cell of 100 mL and extracted with distilled water at temperature of 125°C through two 5 min static cycles, with an 80% flush volume and a 60 s purge. The extracts were lyophilized to obtain the dry extract. Thus, the aqueous extracts of Campomanesia leaves (AECL) and roots (AECR) were obtained with a yield of 13 and 6%, respectively.
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