Adipogenesis Regulation by B. pilosa Extract

Dexamethasone (Dex), insulin, isobutylmethylxanthine (IBMX), Oil Red O powder, rosiglitazone (RGS), sodium bicarbonate, methylthiazoletetrazolium (MTT), Tween 20, isopropanol, formaldehyde, glutamine, glucose, sodium pyruvate, sodium bicarbonate, pantothenic acid, cortisol, triiodothyronine, methanol, and butanol were purchased from Sigma-Aldrich (St. Louis, MO, USA). Penicillin/streptomycin solution, Dulbecco’s Modified Eagle’s Medium (DMEM) and fetal bovine serum (FBS) were obtained from Hyclone Laboratories, Inc. (Logan, UT, USA) and Life Technologies Corp. (Grand Island, NY, USA). B. pilosa extract and GHT were produced in compliance to the good manufacturing practices guidelines and purchased from Chun-Yueh Biomedical Technology Co. (Taipei, Taiwan) as previously described22 (link). Quality control of GHT in each batch of B. pilosa extracts was conducted as shown in Sup. Fig. S3 as previously described22 (link).