Purification and characterization of THU peptides

Acrylamide, nickel chloride, dithiothreitol (DTT), Tris, Luria-Bertani (LB) broth, ampicillin, EDTA, imidazole, isopropyl β-D-1-thiogalactopyranoside (IPTG), phenylmethylsulfonyl fluoride (PMSF), lipopolysaccharide (LPS), MOPS, puromycin, benzamidine, sodium dodecyl sulfate, sodium azide, sodium chloride, TEMED, ammonium persulfate, glycine, and hygromycin B were obtained from Sigma-Aldrich (St. Louis, MO, USA). Chelating Sepharose and SP Sepharose were purchased from GE Healthcare (Uppsala, Sweden). Preparations of tat-derived peptide (GRKKRRQRRR)-His₆-ubiquitin (THU) and THU-RRFKEGGRGGKY (THUC) followed the methods [16 (link)].

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Yu H.C., Huang K.Y., Lu M.C., Huang H.L., Liu S.Q., Lai N.S, & Huang H.B. (2017). Targeted Delivery of the HLA-B∗27-Binding Peptide into the Endoplasmic Reticulum Suppresses the IL-23/IL-17 Axis of Immune Cells in Spondylarthritis. Mediators of Inflammation, 2017, 4016802.

Publication 2017

Acrylamide nickel chloride dithiothreitol ampicillin imidazole isopropyl β-D-1-thiogalactopyranoside phenylmethylsulfonyl fluoride lipopolysaccharide puromycin benzamidine sodium dodecyl sulfate sodium azide sodium chloride TEMED ammonium persulfate glycine hygromycin B Chelating Sepharose SP Sepharose

Acrylamide Ammonium persulfate Ampicillin Benzamidine Dithiothreitol Edta Glycine Hygromycin b Imidazole Lipopolysaccharide Mops Nickel chloride Peptide Phenylmethylsulfonyl fluoride Puromycin Sepharose Sodium azide Sodium chloride Sodium dodecyl sulfate Tris Ubiquitin

Corresponding Organization : National Chung Cheng University

Other organizations : Taipei Tzu Chi Hospital, Fooyin University

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Variable analysis

independent variables

Acrylamide
Nickel chloride
Dithiothreitol (DTT)
Luria-Bertani (LB) broth
Ampicillin
Imidazole
Isopropyl β-D-1-thiogalactopyranoside (IPTG)
Phenylmethylsulfonyl fluoride (PMSF)
Lipopolysaccharide (LPS)
Puromycin
Benzamidine
Sodium dodecyl sulfate
Sodium azide
Sodium chloride
TEMED
Ammonium persulfate
Glycine
Hygromycin B
Tat-derived peptide (GRKKRRQRRR)-His6-ubiquitin (THU)
THU-RRFKEGGRGGKY (THUC)

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

Positive controls: Not specified
Negative controls: Not specified

Annotations

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