Functionalized Gelatin Coatings for Cell Culture

Glass coverslips (Fisher Scientific, 12-545-82) were coated with Oregon Green 488-gelatin (1 mg/ml; Thermo Fisher Scientific, G13186) for 10 min, followed by cross-linking with 0.5% glutaraldehyde (Sigma-Aldrich, G5882) in phosphate-buffered saline (PBS) for 15 min (20 (link)). After that, gelatin-coated coverslips were treated with NaBH₄ (5 mg/ml; Sigma-Aldrich, 452882) for 3 min. Dissociated cells were plated on fluorescent gelatin–coated coverslips and maintained for 3 days before imaging.

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Kwan H.L., Chan Z.C., Bi X., Kutkowska J., Prószyński T.J., Chan C.B, & Lee C.W. (2023). Nerve-independent formation of membrane infoldings at topologically complex postsynaptic apparatus by caveolin-3. Science Advances, 9(24), eadg0183.

Publication 2023

Glass coverslips Oregon Green 488-gelatin NaBH4

Cells Gelatin Glutaraldehyde Oregon green 488 Phosphate Saline

Corresponding Organization : Hong Kong Baptist University

Other organizations : Hong Kong University of Science and Technology, Łukasiewicz Research Network – PORT Polish Center for Technology Development

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Variable analysis

independent variables

Coating of glass coverslips with Oregon Green 488-gelatin (1 mg/ml)
Cross-linking with 0.5% glutaraldehyde in phosphate-buffered saline (PBS) for 15 min
Treatment of gelatin-coated coverslips with NaBH4 (5 mg/ml) for 3 min

dependent variables

Cell behavior/response on fluorescent gelatin-coated coverslips

control variables

Dissociated cells plated on fluorescent gelatin-coated coverslips
Cells maintained for 3 days before imaging

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

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