Protocol detail

Quantifying Choroidal Vascular Density

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Labeled cells were imaged using an LSM 780 confocal microscope (Carl Zeiss). Images were processed using Photoshop CS2 software (Adobe Systems). Choroidal vascular density was analyzed using ImageJ software (NIH) as described previously (Le et al., 2010 (link)). Fluorescence intensity was quantified using Zen Black software (version 2012; Carl Zeiss) according to the manufacturer’s instructions. All images shown are representative of three to eight independent experiments.

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Goto S., Onishi A., Misaki K., Yonemura S., Sugita S., Ito H., Ohigashi Y., Ema M., Sakaguchi H., Nishida K, & Takahashi M. (2018). Neural retina-specific Aldh1a1 controls dorsal choroidal vascular development via Sox9 expression in retinal pigment epithelial cells. eLife, 7, e32358.

Publication 2018

LSM 780 confocal microscope Zen Black software

Cells Choroidal Confocal microscope Fluorescence

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Variable analysis

independent variables

Labeling of cells

dependent variables

Choroidal vascular density
Fluorescence intensity

control variables

Microscope used (LSM 780 confocal microscope)
Image processing software (Photoshop CS2)
Analysis software (ImageJ, Zen Black)

controls

Positive control: Not specified
Negative control: Not specified

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