Intradermal mRNA-LNP Vaccine Tracking

Mice were injected intradermally with 2.5ug mRNA(HA)-LNP or PBS at the upper left site of the back. Two weeks later, the same spot was injected with 2.5ug mRNA(eGFP)-LNP-DiI. Two days post injection, the mice were sacrificed and the skin and skin draining lymph nodes (axillary and brachial) were harvested. Single-cell suspensions were stained with fixable viability dye (Thermo Fisher), anti-CD45 (30 F11), CD11b (M1/70), Ly-6G (1A8), CD64 (X54–5/7.1), CD11c (N418) and MHCII (M5/114.15.2), as we previously described [71 (link)]. The antibodies were purchased from BioLegend, BD Biosciences and Tonbo Biosciences.

Free full text: Click here

Qin Z., Bouteau A., Herbst C, & Igyártó B.Z. (2022). Pre-exposure to mRNA-LNP inhibits adaptive immune responses and alters innate immune fitness in an inheritable fashion. bioRxiv.

Publication Preprint 2022

fixable viability dye

Antibodies Axillary Brachial Cd11b Cell Lymph nodes Mice Mrna Skin

Corresponding Organization : Thomas Jefferson University

Top 5 similar protocols

Variable analysis

independent variables

Injection of 2.5ug mRNA(HA)-LNP or PBS at the upper left site of the back
Injection of 2.5ug mRNA(eGFP)-LNP-DiI at the same spot two weeks later

dependent variables

Cell types in the skin and skin draining lymph nodes (axillary and brachial) measured by flow cytometry, including CD45, CD11b, Ly-6G, CD64, CD11c and MHCII

control variables

Injection site (upper left back)
Dose of mRNA (2.5ug)
Time points (2 weeks and 2 days post-injection)

controls

Positive control: Injection of mRNA(HA)-LNP
Negative control: Injection of PBS

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!