Tumor Spheroid Formation in 3D Petri Dishes

Spheroids were prepared as detailed in our previous work (Steinberg et al. 2020 (link)). Master 3D Petri dish 35-well arrays (Microtissues Inc., Providence, RI, USA) were used to create micro-wells made of 2% UltraPureTM agarose hydrogel (Invitrogen, Carlsbad, CA, USA). Micro-wells were seeded with 5,000 A375 or WM-266-4 cells per well. A375 and WM-266-4 cells were purchased from ATCC. The cells were incubated at 37 °C and 5% CO₂ for 72 h allowing for the growth of tumor spheroids.

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Brill-Karniely Y., Tischenko K, & Benny O. (2023). Analyzing force measurements of multi-cellular clusters comprising indeterminate geometries. Biomechanics and Modeling in Mechanobiology, 23(1), 145-155.

Publication 2023

UltraPureTM agarose hydrogel WM-266-4

Corresponding Organization : Hebrew University of Jerusalem

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Variable analysis

independent variables

Cell type (A375 or WM-266-4 cells)

dependent variables

Growth of tumor spheroids

control variables

Cell seeding density (5,000 cells per well)
Incubation conditions (37 °C, 5% CO2, 72 h)
Culture platform (Master 3D Petri dish 35-well arrays)
Hydrogel material (2% UltraPureTM agarose)

controls

No positive or negative controls were explicitly mentioned.

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