Biphasic calcium phosphate (BCP) particles, constituting 60% hydroxyapatite [HA, Ca10(PO4)6(OH)2] and 40% β-tricalcium phosphate [β-TCP, β-Ca3(PO4)2], were provided by Graftys SA (Aix-en-Provence, France). This CaP ceramic was obtained by sintering (1050 °C for 5 h) calcium-deficient apatite presenting a Ca/P ratio of 1.6 and prepared via an aqueous alkaline hydrolysis method. BCP microparticles measuring 80–200 µm were prepared via crushing and wet sieving, then dried and made endotoxin-free via heating at 250 °C for 1 h. Microparticles were dried and sterilized via heating at 180 °C for 2 h before use.
Free full text: Click here
