Antiviral Compounds Screening Protocol

Remdesivir and Favipiravir were purchased from Carbosynth. TH6744, TH3289 and TH5487 were in-house produced and recently described [28 (link), 29 (link)]. Compounds Camostat (SML0057), E-64d (E8640), Cathepsin L inhibitor (SCP0110) and Bafilomycin A (B1793) were purchased from Sigma-Aldrich. Three phenotypic reference chemicals that are known to produce morphological phenotypes in a variety of cell lines using the Cell Painting assay were included: Etoposide (E1383), Fenbendazole (F5396) and Metoclopramide (M0763) [24 (link), 25 (link)]. DMSO (D2438) was used as compound vehicle (all purchased from Sigma Aldrich).

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Rietdijk J., Tampere M., Pettke A., Georgiev P., Lapins M., Warpman-Berglund U., Spjuth O., Puumalainen M.R, & Carreras-Puigvert J. (2021). A phenomics approach for antiviral drug discovery. BMC Biology, 19, 156.

Publication 2021

Remdesivir Favipiravir Camostat Bafilomycin A DMSO (

Assay Bafilomycin a Camostat Cathepsin l Cell Cell lines Dmso E 64d Etoposide Favipiravir Fenbendazole Metoclopramide Phenotypes Remdesivir Th5487

Corresponding Organization :

Other organizations : Science for Life Laboratory, Uppsala University, Karolinska Institutet

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Variable analysis

independent variables

Remdesivir
Favipiravir
TH6744
TH3289
TH5487
Camostat
E-64d
Cathepsin L inhibitor
Bafilomycin A
Etoposide
Fenbendazole
Metoclopramide

dependent variables

Morphological phenotypes in a variety of cell lines using the Cell Painting assay

control variables

DMSO (D2438) used as compound vehicle

controls

Positive controls: Etoposide, Fenbendazole, Metoclopramide (known to produce morphological phenotypes in a variety of cell lines using the Cell Painting assay)
Negative control: DMSO (compound vehicle)

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