The ADI modification by BD was carried out at room temperature according to Bihzad and El-Shora [33 (link)]. The butanedione-borate solution was tested at different concentrations (2, 4, 6, 8 and 10 mM). Aliquot from each concentration was mixed with 10 ml of ADI in 50 mM sodium borate buffer (pH 7.0) to initiate the modification reaction. ADI activity was determined immediately afterwards.
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