BrdU/EdU double-labeling was carried out according to Houlihan et al. (37 (link)). Briefly, pregnant females were injected intraperitoneally with BrdU, (Millipore-Sigma) (50 mg/kg body weight) and 1.5 hours later with the same dose of 5-Ethynyl-2’- deoxyuridine (EdU, Millipore-Sigma) and sacrificed after 0.5 hours. For EdU single-labeling, pregnant mice were injected intraperitoneally with EdU (50 mg/kg body weight) at E14.5 and sacrificed after 0.5 hours, 24 hours, or 4 days. The obtained mouse brain slices were stained for Edu immunohistochemistry using the Click-iT Edu kit (C10338,Thermo Fisher Scientific).
Chen G., Yu B., Tan S., Tan J., Jia X., Zhang Q., Zhang X., Jiang Q., Hua Y., Han Y., Luo S., Hoekzema K., Bernier R.A., Earl R.K., Kurtz-Nelson E.C., Idleburg M.J., Madan-Khetarpal S., Clark R., Sebastian J., Fernandez-Jaen A., Alvarez S., King S.D., Ramos L.L., Santos M.L., Martin D.M., Brooks D., Symonds J.D., Cutcutache I., Pan Q., Hu Z., Yuan L., Eichler E.E., Xia K, & Guo H. (2022). GIGYF1 disruption associates with autism and impaired IGF-1R signaling. The Journal of Clinical Investigation, 132(19), e159806.
Corresponding Organization : Central South University
Other organizations :
University of Washington, Children's Hospital of Pittsburgh, University of Pittsburgh Medical Center, University of Pittsburgh, Hospital Universitario Quirónsalud Madrid, Universidad Europea de Madrid, Baylor College of Medicine, Mendelics, Universidade de São Paulo, Hospital Erasto Gaertner, Michigan Medicine, Royal Hospital for Children, UCB Pharma (United Kingdom), Howard Hughes Medical Institute, University of South China, Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences
Time of BrdU injection (1.5 hours before sacrifice)
Time of EdU injection (0.5 hours before sacrifice)
Time of EdU injection (E14.5, 0.5 hours, 24 hours, or 4 days before sacrifice)
dependent variables
Presence and localization of BrdU and EdU labeling in mouse brain slices
control variables
Mouse strain
Gestational stage (E14.5)
controls
Positive control: None specified
Negative control: None specified
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to
get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
