Arabidopsis thaliana Columbia (Col-0) ecotype was used as background for mutants and transgenic lines. cry1-30494 (link), cry2-191 (link)cry1-304 cry2-194 (link), ubp12-2w ubp13-336 (link), ku7CP95 (link), cop1-496 (link) and camta12397 (link) mutants have been previously described. Col-0;UBQ10pro:UBP13-6xHA (UBP13oe)25 (link), cry2-1;UBQ10pro:UBP13-6xHA;CRY2pro:2xStrep-6xHis-3xFLAG-CRY225 (link), ubp12-2w ubp13-3;UBQ10pro: 2xStrep-6xHis-3xFLAG-CRY2 (ubp12ubp13;CRY2oe)25 (link), Col-0;UBQ10pro:COP1-6xHis-3xFLAG (COP1oe)25 (link), and cry2-1;UBQ10pro:2xStrep-6xHis-3xFLAG-CRY2 (cry2;CRY2oe)98 (link) lines have been described previously. After surface sterilization, seeds were plated on 0.5× Linsmaier and Skoog (LS) medium (HiMedia Laboratories) containing 0.8% agar, stratified for 2 days in darkness at 4°C and then grown at 22°C under 100 μmol m−2 s−1 white light from a LED source in a growth chamber (Percival Scientific) unless otherwise specified.
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Arabidopsis mutant and transgenic lines
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Corresponding Organization : Cold Spring Harbor Laboratory
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Variable analysis
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cry1-304 cry2-1 cry1-304 cry2-1 ubp12-2w ubp13-3 ku7CP cop1-4 camta123 Col-0;UBQ10pro:UBP13-6xHA (UBP13oe) cry2-1;UBQ10pro:UBP13-6xHA;CRY2pro:2xStrep-6xHis-3xFLAG-CRY2 ubp12-2w ubp13-3;UBQ10pro: 2xStrep-6xHis-3xFLAG-CRY2 (ubp12ubp13;CRY2oe) Col-0;UBQ10pro:COP1-6xHis-3xFLAG (COP1oe) cry2-1;UBQ10pro:2xStrep-6xHis-3xFLAG-CRY2 (cry2;CRY2oe)
- Not explicitly mentioned
Arabidopsis thaliana Columbia (Col-0 ) ecotype- Surface sterilization of seeds
- Plating seeds on 0.5× Linsmaier and Skoog (LS) medium with 0.8% agar
- Stratification for 2 days in darkness at 4°C
- Growth at 22°C under 100 μmol m−2 s−1 white light from a LED source in a growth chamber
Arabidopsis thaliana Columbia (Col-0 ) ecotype as background for mutants and transgenic lines
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