Rd10 nontreated and rd10+PTZ mice were anesthetized with ketamine/xylazine as described.16 (link) Retinal structure was evaluated in vivo using a Bioptigen Spectral Domain Ophthalmic Imaging System (SDOIS; Bioptigen Envisu R2200, Morrisville, NC, USA) in mice at ages P60, P90, P120, and P180. The imaging protocol included averaged single B-scan and volume intensity scans with images centered on the optic nerve head. Because of the considerable outer retinal disruption in rd10 retina, it is preferable to use the manual caliper feature to measure retinal layers versus autosegmentation postimaging analysis (a feature of the InVivoVue Diver 2.4 software). We used the manual caliper feature to acquire inner, outer, and total retinal thickness measurements. Inner retina was measured from the superior boundary of inner limiting membrane (ILM) to the lower edge of the inner nuclear layer (INL). Outer retina was measured from the lower edge of the INL to the inferior boundary of the retinal pigment epithelium layer (RPE); if there was separation of photoreceptors from RPE, we obtained outer retinal thickness by adding the RPE layer thickness and the distance from the lower edge of INL to the edge of neuronal retina as described previously.22 (link)