Murine Allergic Asthma Induction and Treatment

Mice were sensitized with 100 µg of Grade V chicken OVA (Sigma-Aldrich, St. Louis MO) mixed with 2 mg aluminum hydroxide in saline by intraperitoneal (i.p.) injection twice, once a week as previously described [16 (link), 17 (link)]. Mice were then challenged with aerosolized 3% OVA for 30 min once for the acute model or 3 times per week for 4 weeks for the chronic model. Administration i.p. of S-NACH (water soluble) or vehicle (saline) was done 30 min after the OVA challenge. Mice were sacrificed 48 h later for bronchoalveolar lavage (BAL) or lung fixation and processing. Some mice were subjected to AHR measurements 24 h after the OVA challenge as described [16 (link), 17 (link)].