The STAT3 inhibitor StatticĀ® was prepared and applied prior to vaccinia scarification as previously described [4 (link)]. Cyclophosphamide (Santa Cruz Biotech, Dallas, TX) was administered by intraperitoneal route starting with the day prior to vaccinia scarification and every 4 days after vaccinia infection as previously described [8 (link)]. Scarification using 106 pfu ACAM-2000 was performed as previously described [4 (link)].
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