For fluorescence and light microscopic analysis, 50 µl AMM on glass coverslips in a wet chamber was inoculated with 2 × 104 conidia. Samples were analyzed after 0, 4, 6, 10, and 24 h using a Zeiss Axio Imager.M2 (Zeiss). Images were taken with an AxioCam MRm and analyzed by the use of AxioVision SE64 Rel. 4.9.1 imaging software (Zeiss). For confocal scanning laser microscopy, a Zeiss LSM 780 instrument was used along with an Airyscan detector where noted. Images were processed using the ZEN software package from Zeiss.
For scanning electron microscopy (SEM) analysis, resting conidia from mycelia grown on AMM agar plates for 5 days were collected using an electrically conductive and adhesive tag (Leit-Tab; Plano GmbH). Samples for resting conidia were fixed for 24 h in a desiccator containing a solution of 25% (vol/vol) glutaraldehyde, whereas swollen conidia were fixed in 2.5% (vol/vol) glutaraldehyde. Further preparation and scanning electron microscopy were carried out as previously described (56 (link)).
For scanning electron microscopy (SEM) analysis, resting conidia from mycelia grown on AMM agar plates for 5 days were collected using an electrically conductive and adhesive tag (Leit-Tab; Plano GmbH). Samples for resting conidia were fixed for 24 h in a desiccator containing a solution of 25% (vol/vol) glutaraldehyde, whereas swollen conidia were fixed in 2.5% (vol/vol) glutaraldehyde. Further preparation and scanning electron microscopy were carried out as previously described (56 (link)).
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