Two-Photon Imaging of Anesthetized Mice

Two-photon imaging was performed on urethane-anesthetized adult mice (as above) using a resonant scanner-based B-Scope (Thorlabs) with a Chameleon Vision 2 laser (coherent, wavelength 920 nm) and an Olympus objective lens (XLPlan N 25×) as described before^{18 ,26 (link)}. The B-Scope is equipped with a reverse dichroic mirror (ZT405/488/561/680-1100rpc, Chroma) and the emission light was separated by a dichroic mirror (FF562-Di03, Semrock) with bandpass filters FF03-525/50 and FF01-607/70 (both from Semrock) for the green and red channels, respectively. Images were acquired using the ThorImage software at a frame rate of 30 Hz.

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Monai H., Koketsu S., Shinohara Y., Ueki T., Kusk P., Hauglund N.L., Samson A.J., Nedergaard M, & Hirase H. (2021). Adrenergic inhibition facilitates normalization of extracellular potassium after cortical spreading depolarization. Scientific Reports, 11, 8150.

Publication 2021

FF562-Di03 FF03-525/50 FF01-607/70

Adult Chameleon Frame Lens Light Mice Urethane Vision

Corresponding Organization :

Other organizations : RIKEN Center for Brain Science, Nagoya City University, University of Copenhagen

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Two-photon imaging

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Urethane-anesthetized adult mice
Resonant scanner-based B-Scope (Thorlabs) with a Chameleon Vision 2 laser (coherent, wavelength 920 nm) and an Olympus objective lens (XLPlan N 25×)
Reverse dichroic mirror (ZT405/488/561/680-1100rpc, Chroma)
Dichroic mirror (FF562-Di03, Semrock) with bandpass filters FF03-525/50 and FF01-607/70 (both from Semrock) for the green and red channels, respectively
Frame rate of 30 Hz

controls

No positive or negative controls are explicitly mentioned.

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