Targeted RNAi Silencing of Chromatin Regulators

RNAi was performed by the transfection of siRNA oligos using the Lipofectamine RNAiMAX transfection reagent (Thermo Fisher Scientific), according to the manufacturer’s instructions. The sequence of siRNA oligos was as followed.
BRD2 (BRD2, Thermo Fisher Scientific, validated Silencer Select siRNA, ID: s12071). BRD3 (BRD3, Thermo Fisher Scientific, validated Silencer Select siRNA, ID: s15544^{48 (link)}). BRD4_1 (BRD4, Thermo Fisher Scientific, validated Silencer Select siRNA, ID: S23901^{48 (link)}). BRD4_2 (BRD4, Dharmacon, siGENOME siRNA, ID: M-004937-02-0005). BRD4_short (BRD4, Qiagen, ID: SI05044865^{22 (link)}). BRD4_long (BRD4, Qiagen, ID: SI05044872^{22 (link)}). XRCC4_1 (XRCC4, Sigma Aldrich, Mission siRNA, ID: SASI_Hs01_00114717). XRCC4_2 (XRCC4, Dharmacon, ON-TARGET plus siRNA, ID: L-004494-00-0005^{49 (link)}). 53BP1_1 (53BP1, Sigma Aldrich, Mission siRNA, ID: SASI_Hs02_00340027). 53BP1_2 (53BP1, Dharmacon, ON-TARGET plus siRNA, ID: L-003548-00-0005^{50 (link)}).

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Wakita M., Takahashi A., Sano O., Loo T.M., Imai Y., Narukawa M., Iwata H., Matsudaira T., Kawamoto S., Ohtani N., Yoshimori T, & Hara E. (2020). A BET family protein degrader provokes senolysis by targeting NHEJ and autophagy in senescent cells. Nature Communications, 11, 1935.

Publication 2020

Silencer Select siRNA siGENOME siRNA XRCC4 Mission siRNA ON-TARGET plus siRNA

53bp1 Brd4 Lipofectamine Oligos Rnai Sirna Transfection Xrcc4

Corresponding Organization : Japanese Foundation For Cancer Research

Other organizations : Takeda (Japan), Osaka City University

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Variable analysis

independent variables

SiRNA oligos for BRD2
SiRNA oligos for BRD3
SiRNA oligos for BRD4 (BRD4_1, BRD4_2, BRD4_short, BRD4_long)
SiRNA oligos for XRCC4 (XRCC4_1, XRCC4_2)
SiRNA oligos for 53BP1 (53BP1_1, 53BP1_2)

dependent variables

Not explicitly mentioned

control variables

Lipofectamine RNAiMAX transfection reagent used for the transfection of siRNA oligos

controls

No positive or negative controls were explicitly mentioned

Annotations

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