Eosinophil Infiltration and Degranulation Assessment

RNA was extracted from full thickness mucosa or LSM as previously described^{13 (link)}. Paraffin embedded full thickness mucosa was used in immunohistochemistry as previously described^{13 (link)}. Infiltrating intact eosinophils and eosinophil degranulation (i.e., the presence of free cytoplasmic granules and/or tissue deposition of eosinophil granule proteins) were assessed by immunohistochemistry using a mouse monoclonal anti-eosinophil peroxidase antibody (EPX-mAb) as previously described^{38 (link),39}. Tissue sections were digitized (Aperio AT Turbo, Leica Biosystems, Buffalo Grove, IL) and images were generated using Aperio ImageScope software (version 11.2.0.780, Aperio Technologies, Vista, CA).

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Kurten R.C., Rawson R., Shoda T., Duong L.D., Adejumobi D., Levy R., Newbury R.O., Rothenberg M.E., Akuthota P., Wright B.L., Dohil R., Jones S.M, & Aceves S.S. (2019). Development and Application of a Functional Human Esophageal Mucosa Explant Platform to Eosinophilic Esophagitis. Scientific Reports, 9, 6206.

Publication 2019

Aperio AT Turbo Aperio ImageScope software

Anti antibody Buffalo Cytoplasmic granules Eosinophil Eosinophil granule proteins Eosinophil peroxidase Immunohistochemistry Mouse Mucosa Paraffin Tissue

Corresponding Organization :

Other organizations : University of Arkansas for Medical Sciences, Rady Children's Hospital-San Diego, University of California, San Diego, Cincinnati Children's Hospital Medical Center, Arkansas Children's Hospital, University of California System, Phoenix Children's Hospital, Mayo Clinic in Florida

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Variable analysis

independent variables

RNA extraction from full thickness mucosa or LSM
Immunohistochemistry using a mouse monoclonal anti-eosinophil peroxidase antibody (EPX-mAb)

dependent variables

Infiltrating intact eosinophils
Eosinophil degranulation (i.e., the presence of free cytoplasmic granules and/or tissue deposition of eosinophil granule proteins)

control variables

Paraffin embedded full thickness mucosa

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

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