Evaluating rTMS Effects on Cerebral Microcirculation

To evaluate the effect of rTMS on microcirculation, LSI was carried out as described previously (Beker et al., 2015 (link)) with minor modifications. Briefly, mice were exposed to 90 min MCAo as described above. Starting 1 min after reperfusion, real-time CBF changes were recorded by Pericam PSI System (Perimed). To evaluate the CBF changes in the ischemic core and ischemic penumbra regions of interest (ROI) covering 1.0 mm × 5.5 mm (in lateral and rostrocaudal direction, respectively) were defined 0.5 and 1.5 lateral and 0.5 mm posterior to the bregma, in which mean CBF was calculated using a blood perfusion imaging software (PIMSoft; Perimed). Regional CBF was recorded throughout the 60 min observation period after rTMS treatment. From the measurements obtained, relative CBF changes (in %) at the end of the observation period which is compared with the values of before rTMS treatment after MCAo.

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Caglayan A.B., Beker M.C., Caglayan B., Yalcin E., Caglayan A., Yulug B., Hanoglu L., Kutlu S., Doeppner T.R., Hermann D.M, & Kilic E. (2019). Acute and Post-acute Neuromodulation Induces Stroke Recovery by Promoting Survival Signaling, Neurogenesis, and Pyramidal Tract Plasticity. Frontiers in Cellular Neuroscience, 13, 144.

Publication 2019

Pericam PSI System PIMSoft

Blood Mice Microcirculation Reperfusion Rtms

Corresponding Organization :

Other organizations : Regenerative Medicine Institute, Istanbul Medipol University, Necmettin Erbakan University, University of Göttingen, University of Duisburg-Essen

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Variable analysis

independent variables

RTMS treatment

dependent variables

Relative CBF changes (in %) at the end of the observation period compared to before rTMS treatment after MCAo

control variables

Mice exposed to 90 min MCAo
Real-time CBF changes recorded by Pericam PSI System (Perimed)
ROI covering 1.0 mm × 5.5 mm (in lateral and rostrocaudal direction, respectively) defined 0.5 and 1.5 lateral and 0.5 mm posterior to the bregma
Mean CBF calculated using a blood perfusion imaging software (PIMSoft; Perimed)

controls

Positive control: Not specified
Negative control: Not specified

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