Transgenic Mouse Models Overexpressing BMF and hnRNP F

Tg mice specifically overexpressing human myc-BMF in their RPTCs were generated using a similar strategy to that described previously^{20 (link),28 (link),49 (link)}. In brief, full-length human BMF cDNA fused with Myc-tag was inserted into pKAP2 plasmid at the NotI site. The plasmid pKAP2 containing the KAP promoter that is responsive to androgen was a gift from Dr. Curt D. Sigmund (University of Iowa, Iowa City, IA)^{50 (link)}.
Akita hnRNP F-Tg mice were generated by cross-breeding hnRNP F-Tg mice with heterozygous Akita (C57BL/6-Ins2 Akita/J) mice (Jackson Laboratory, Ann Harbor, ME) as previously described^{28 (link)}.

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Ghosh A., Zhao S., Lo C.S., Maachi H., Chenier I., Lateef M.A., Abdo S., Filep J.G., Ingelfinger J.R., Zhang S.L, & Chan J.S. (2019). Heterogeneous Nuclear Ribonucleoprotein F Mediates Insulin Inhibition of Bcl2-Modifying Factor Expression and Tubulopathy in Diabetic Kidney. Scientific Reports, 9, 6687.

Publication 2019

C57BL/6-Ins2 Akita/J) mice

Androgen Cdna Heterozygous Hnrnp f Human Mice Plasmid

Corresponding Organization :

Other organizations : Centre Hospitalier de l’Université de Montréal, Hôpital Maisonneuve-Rosemont, Université de Montréal, Massachusetts General Hospital, Harvard University

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independent variables

Overexpression of human myc-BMF in RPTCs of Tg mice

dependent variables

Not explicitly mentioned

control variables

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controls

Positive control: Not mentioned
Negative control: Not mentioned

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