Naive CD4+ T cells isolated from WT and Cblb−/− mice were stimulated with plate-bound anti-CD3 (2 μg/ml) and anti-CD28 (1 μg/ml) in the presence of Th1, Th2, Th17, or Th9 cytokine cocktails as previously described (Ying et al., 2010 ; Chang et al., 2010 ). Details are provided in the Supplemental Experimental Procedures.
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