Olaparib and Cisplatin Cytotoxicity Evaluation

Olaparib (ASD2281, Selleck Chemicals LCC, Houston, TX) was dissolved in molecular-grade water at a concentration of 10 mM. For cell viability assays, the measurements were performed after treatment with different concentrations (0, 5, 10, 15, 20, 30, 40, and 50 µM) of Olaparib for 48 h, as previously described [12 (link), 18 (link)]. For specific assays, cell lines were incubated with Olaparib 10 µM for 24 and 48 h. Cisplatin (S116650MG, Selleck Chemicals) was dissolved in molecular-grade water at a concentration of 10 mM. For specific assays, cell lines were incubated with Cisplatin for 12 and 24 h.

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Bustos M.A., Yokoe T., Shoji Y., Kobayashi Y., Mizuno S., Murakami T., Zhang X., Sekhar S.C., Kim S., Ryu S., Knarr M., Vasilev S.A., DiFeo A., Drapkin R, & Hoon D.S. (2023). MiR-181a targets STING to drive PARP inhibitor resistance in BRCA- mutated triple-negative breast cancer and ovarian cancer. Cell & Bioscience, 13, 200.

Publication 2023

Olaparib Cisplatin

After treatment Assays Cell lines Cell viability Cisplatin Olaparib

Corresponding Organization :

Other organizations : Saint John's Health Center, University of Michigan–Ann Arbor, University of Pennsylvania

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Variable analysis

independent variables

Olaparib concentration (0, 5, 10, 15, 20, 30, 40, and 50 µM)
Olaparib incubation time (24 h and 48 h)
Cisplatin incubation time (12 h and 24 h)

dependent variables

Cell viability

control variables

Cell lines
Solvent (molecular-grade water)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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