Cultivation and Stress Exposures of Bacterial Strains

Bacterial strains, plasmids and primers used in this study are listed in Supplementary Tables S1, S2, S3. For cloning and genetic manipulation, Escherichia coli was cultivated in LB medium. For stress experiments, S. aureus COL wild type and mutant strains were cultivated in LB, RPMI, or Belitsky minimal medium and exposed to the different compounds during the exponential growth as described previously (Loi et al., 2017 (link), 2018b (link)). NaOCl, methylglyoxal, diamide, methylhydroquinone, DTT, cumene hydroperoxide (80% w/v), H₂O₂ (35% w/v), and monobromobimane were purchased from Sigma Aldrich.

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Linzner N., Loi V.V., Fritsch V.N., Tung Q.N., Stenzel S., Wirtz M., Hell R., Hamilton C.J., Tedin K., Fulde M, & Antelmann H. (2019). Staphylococcus aureus Uses the Bacilliredoxin (BrxAB)/Bacillithiol Disulfide Reductase (YpdA) Redox Pathway to Defend Against Oxidative Stress Under Infections. Frontiers in Microbiology, 10, 1355.

Publication 2019

NaOCl methylglyoxal diamide methylhydroquinone DTT cumene hydroperoxide monobromobimane

Bacterial Cumene hydroperoxide Diamide Escherichia coli H2o2 Manipulation Methylglyoxal Monobromobimane Plasmids Primers S aureus Strains

Corresponding Organization : Freie Universität Berlin

Other organizations : Heidelberg University, University of East Anglia

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Variable analysis

independent variables

NaOCl
Methylglyoxal
Diamide
Methylhydroquinone
Cumene hydroperoxide (80% w/v)
H2O2 (35% w/v)
Monobromobimane

dependent variables

Bacterial growth and survival under stress conditions

control variables

Escherichia coli was cultivated in LB medium
S. aureus COL wild type and mutant strains were cultivated in LB, RPMI, or Belitsky minimal medium

positive controls

Not mentioned

negative controls

Not mentioned

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