Gamma Irradiation of Arabidopsis Seeds

Dry seeds of Arabidopsis thaliana ecotype Landsberg (Ler) were irradiated with ⁶⁰Co gamma irradiator (150 TBq of capacity, Nordion, Ottawa, ON, Canada) for 8 h at the Korea Atomic Energy Research Institute [64 (link)]. First, the seeds (first batch) for preliminary image analysis of phenotypic variation among treatments were prepared using four doses of gamma rays (200, 400, 600, and 800 Gy). Second, the seeds (second batch) for the main test of small phenotypic variation detection among treatments were prepared using four smaller doses of gamma rays (100, 200, 300, and 400 Gy). The second batch of irradiated seeds was used for validation study. All irradiated seeds were stored at 4 °C until further analysis.