Validating Hub RBPs Expression and Prognosis

In order to verify the prognosis of hub RBPs, we used Kaplan-Meier Plotter (http://kmplot.com/) to verify it in the GSE29272 data set 24 (link). For the verification of hub RBPs expression, we first use the GEPIA network tool for verification, which contains data from the TCGA and GTEx databases 25 (link). In addition, we also collected surgical samples from 10 pairs of gastric cancer patients. The process was approved by the patient's informed consent and the ethics committee of the Second Affiliated Hospital of Nanchang University. After homogenizing the clinical samples, the Trizol (Thermo Fisher, USA) method was used to extract total RNA. The obtained RNA was reverse transcribed using reverse transcription kit RR047A (Takara, Japan). ACTB was used as the internal reference gene, and the mRNA expression of hub RBPs was analyzed by fluorescence quantitative PCR using the RR820 kit (Takara, Japan) on the 7900-HT system (Thermo Fisher, USA). The primers used are all synthesized by Shanghai Shenggong Company, and the sequences of all primers are in Supplementary Table 2.