Characterizing Cellular Differentiation via IF/IHC

Immunofluorescence (IF) and immunohistochemistry (IHC) were performed as previously described in ref. ^{45 (link)}. Primary antibodies included anti-Nestin (Proteintech, 19483-1-AP, 1:200), anti-CUL4B (Sigma, C9995, 1:200), anti-OCN (Abcam, ab93876, 1:200) and anti-FABP4 (Abcam, ab92501, 1:200). Secondary antibodies included goat anti-rabbit or mouse horseradish peroxidase (HRP) and goat anti-rabbit or mouse Cy2 or Cy3 (Jackson ImmunoResearch, 1:200). Integral optical density (IOD) in IHC was quantified by ImageJ.

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Yu R., Han H., Chu S., Ding Y., Jin S., Wang Y., Jiang W., Liu Y., Zou Y., Wang M., Liu Q., Sun G., Jiang B, & Gong Y. (2023). CUL4B orchestrates mesenchymal stem cell commitment by epigenetically repressing KLF4 and C/EBPδ. Bone Research, 11, 29.

Publication 2023

ab93876 ab92501

Antibodies Fabp4 Goat Horseradish peroxidase Immunofluorescence Immunohistochemistry Mouse Nestin Rabbit

Corresponding Organization :

Other organizations : Shandong University

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Variable analysis

independent variables

Primary antibodies: anti-Nestin, anti-CUL4B, anti-OCN, anti-FABP4

dependent variables

Integral optical density (IOD) in IHC quantified by ImageJ

control variables

Immunofluorescence (IF) and immunohistochemistry (IHC) protocols were performed as previously described in ref. 45 (link)
Secondary antibodies: goat anti-rabbit or mouse horseradish peroxidase (HRP) and goat anti-rabbit or mouse Cy2 or Cy3 (Jackson ImmunoResearch, 1:200)

controls

Positive controls: Not explicitly mentioned
Negative controls: Not explicitly mentioned

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