Plasma Biomarker Quantification in Down Syndrome

Plasma p-tau217 concentration was measured according to the published protocols using immunoassay on a Mesoscale Discovery platform developed by Lilly Research Laboratories.^{17 (link),30 (link)} Briefly, biotinylated-IBA493 was used as a capture antibody and SULFO-TAG-4G10-E2 (anti-Tau) as the detector, and samples were diluted 1:2. The assay was calibrated with a synthetic p-tau217 peptide. Plasma GFAP concentration was quantified using a Simoa kit (Quanterix) according to the manufacturer’s instructions. Plasma Aβ42/Aβ40, NfL, and t-tau were analyzed with a Simoa 4-plex kit (Quanterix) in plasma samples from 258 participants with DS and 28 non-DS siblings as previously described.^{12 (link)} Plasma sampling and analysis are further described in the eMethods in the Supplement. All samples were analyzed by staff who were blinded to the clinical and imaging data.

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Janelidze S., Christian B.T., Price J., Laymon C., Schupf N., Klunk W.E., Lott I., Silverman W., Rosas H.D., Zaman S., Mapstone M., Lai F., Ances B.M., Handen B.L, & Hansson O. (2022). Detection of Brain Tau Pathology in Down Syndrome Using Plasma Biomarkers. JAMA Neurology, 79(8), 797-807.

Publication 2022

Antibody Assay Gfap Immunoassay Peptide p Plasma Siblings Supplement

Corresponding Organization : University of Pittsburgh

Other organizations : University of Wisconsin–Madison, Massachusetts General Hospital, Harvard University, Columbia University, University of California, Irvine, University of Cambridge, Washington University in St. Louis, Skåne University Hospital

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Plasma p-tau217 concentration
Plasma GFAP concentration
Plasma Aβ42/Aβ40 ratio
Plasma NfL concentration
Plasma t-tau concentration

control variables

Plasma samples from 258 participants with Down syndrome and 28 non-DS siblings

positive controls

Synthetic p-tau217 peptide used for assay calibration

negative controls

None explicitly mentioned

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