Human adipose derived stem cells (hADSC) were delivered by iXCells Biotechnologies (San Diego, USA, Cat. Nr. 10HU-001). RPMI 1640 medium was obtained from GIBCO (Thermo Fisher Scientific, Schwerte, Germany). Fetal bovine serum (FBS) was provided by HyClone Laboratories Inc. (Bath, UK). The sources of the phospholipases and antibodies (including the dilutions used) were given previously [53 (link)] unless indicated otherwise. 1-ethyl-3-[3-dimethylaminopropyl] carbodiimide (EDC) and N-hydroxysulfosuccinimide (Sulfo-NHS, premium grade) were bought from Pierce/Thermo Scientific (Rockford, IL, USA).
Protein A-Sepharose (Cl-4B) and phenylsepharose were from Calbiochem/Merck (Darmstadt, Germany). Polystyrene Bio-Beads SM-2 (20–50 mesh) were bought from Bio-Rad Laboratories (Munich, Germany). Mannosamine (ManN, 2-amino-2-deoxy D-mannose), methyl-ß-cyclodextrin (mßCD), BSA (fraction V, defatted) and human serum (normal healthy probands) were delivered by Sigma-Aldrich (Deisenhofen, Germany). Human (recombinant) insulin was a kind gift from Sanofi Pharma Germany GmbH (Diabetes group, Frankfurt am Main, Germany). Other materials (highest purity available) were obtained as described previously [31 (link),32 (link),53 (link),82 (link),83 (link)].
Protein A-Sepharose (Cl-4B) and phenylsepharose were from Calbiochem/Merck (Darmstadt, Germany). Polystyrene Bio-Beads SM-2 (20–50 mesh) were bought from Bio-Rad Laboratories (Munich, Germany). Mannosamine (ManN, 2-amino-2-deoxy D-mannose), methyl-ß-cyclodextrin (mßCD), BSA (fraction V, defatted) and human serum (normal healthy probands) were delivered by Sigma-Aldrich (Deisenhofen, Germany). Human (recombinant) insulin was a kind gift from Sanofi Pharma Germany GmbH (Diabetes group, Frankfurt am Main, Germany). Other materials (highest purity available) were obtained as described previously [31 (link),32 (link),53 (link),82 (link),83 (link)].
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