High performance liquid chromatography mass spectrometry was used to analyze AHL production by YZ1 and YZ2 using a LCQ Deca XP max (Thermo Finnigan, USA; Yin et al., 2012 (link); Chen et al., 2013 (link)). Liquid chromatography steps utilized a C18 column of 4.6 × 150 mm, 5 μm particle size, with injection volume of 20 μl and a flow rate of 0.3 ml/min. Mobile phases were 0.1% v/v formic acid in water and 0.1% v/v formic acid in acetonitrile, respectively. Mass spectrometry was run in ESI-positive mode, with probe capillary voltage set at 3,000 V, a desolvation temperature of 350°C, sheath gas of 11 ml/h, and a nebulizer pressure of 50 psi (Chen et al., 2013 (link)).
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