Melatonin was extracted according to our previous study18 (link). The samples were separated on a Waters Acquity UHPLC system (Milford, MA, USA) equipped with a BEH C18 column (Waters, 2.1 mm internal diameter ×50 mm length, and 1.7 µm particle size). Mass spectrometry (MS) analyses were performed using a QTof-Micro mass spectrometer. The detection parameters and conditions of the ultra-high-performance liquid chromatography (UHPLC)-MS analysis were set according to our previous study26 (link).
The ethylene production rate was measured as described in a previous study18 (link). ACC extraction and determination were performed according to the method described by Tucker et al.59 (link).
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