After different oxygen treatment, the radioresistant cells were harvested and fixed in 70% ice cold ethanol and followed by RNase A treatment, stained with 50 lg/mL of propidium iodide for the DNA content analysis by flow cytometry on a FACS Calibur system (EPICS ALTRA, Beckman Coulter, Fullerton, CA). The data were processed by FlowJo FACS analysis software (Tree Star, Ashland, OR) [16 (link)].
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