Lentiviral infections and plasmid transfections were performed as previously described (Xi et al. 2011 (link)). To generate plasmids for doxycycline-inducible vectors, the ORFs of SMAD2 and SMAD2β were cloned into pLVX-Tight-Puro vector (Clontech), respectively, and HA- tag was added at the N-terminal accordingly. In addition, the CMV promoter present in plasmid pLVX-Tet-On was replaced with a pGK promoter to avoid silencing in mESCs.
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