LGG administration was performed as previously described.24 (link) Mice were orally inoculated with LGG (ATCC 53103) (Bayer) (5 × 109 CFU per 200 μl of saline) daily for 7 d and then orally once every 3 d until the end of the experiment according to the manufacturer’s instructions.
For butyrate administration, butyrate (Sigma-Aldrich) was dissolved in saline and diluted in drinking water at a final concentration of 6 mol/L for gavage. Drinking water containing same concentration of saline was prepared as control solution. butyrate group and control group were administered continuously by daily gavage of 150 μl of prepared butyrate solution or control solution for 8 d.
For butyrate administration, butyrate (Sigma-Aldrich) was dissolved in saline and diluted in drinking water at a final concentration of 6 mol/L for gavage. Drinking water containing same concentration of saline was prepared as control solution. butyrate group and control group were administered continuously by daily gavage of 150 μl of prepared butyrate solution or control solution for 8 d.
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